Negative Hydrogen Ions are studied extensively, especially in Japan, for their therapeutic effects as antioxidants. We have compiled a list of research that show the health benefits of these negative hydrogen ions.

Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals
Hydrogen may reduce the risks related to radiation-induced oxidative stress in space flight

1. An examination of the relationship between coral calcium hydride and hormone secretion.

Purpose
Examination of the relationship between coral calcium hydride and hormone secretion.

Method
Subjects were made up of:
5 male athletes (average age 37)
11 female athletes (average age 23)
Subjects consumed 1 capsule of coral calcium hydride per day as a base and 2 capsules before exercise. The participants exercised for one hour. Changes in hormone secretion were examined by blood tests, which covered 15 different items and were administered before and after exercise. We compared results after the 4 th and 12 th week.

Results
1) Estradiol (E2): There was a significant rise in female subjects in the 4 th and 12 th week.
2) Adrenocorticotropic hormone (ATCH): Overall there was a decline of this hormone in male participants but a significant rise in female subjects in the 4 th and 12 th week.
3) Catecholamines: Adrenaline rose significantly in male subjects in the 4 th week. In female subjects adrenaline rose significantly in the 4 th and 12 th weeks. Noradrenalin rose significantly in female subjects in the 12 th week.

Conclusion
The stress hormone and sex hormone reactions, especially with female subjects, may be the result of coral calcium hydride’s effect on ATP production

2. The physical property of water is changed by metal hydride and achieves stable hydrogen saturation.

Purpose
Awareness of the antioxidant function of hydrogen has grown recently. However, it is difficult to store hydrogen in water stably. We examined the question of hydrogen stability in water.

Method
e combined ordinary tap water with the coral calcium metal hydride substance. We then infused hydrogen gas into the water until saturation. OPR and pH were then measured chronologically against the level of dissolved hydrogen.

Results
After 12 hours the amount of dissolved hydrogen gas in normal water had dissipated to zero. However, in water containing metal hydride it took 20 hours for the dissolved hydrogen to reach zero and then after 84 hours hydrogen again started to be emitted. This hydrogen emission continued for over a week.

Conclusion
The physical property of water changes when metal hydride is placed into it. Alkaline reduction ionic mineral water is produced, which is close to oxygen- free. It is theorized that hydrogen effectively enters a plasma state, which enables hydrogen to be stabilized within the water.

3. The effects of coral calcium hydride on human hyperlipemia

Purpose
We examined the effects of orally ingestible coral calcium hydride (referred to as CCH) on human hyperlipemia.

Method
After providing dietary guidance to 10 adult male and female patients with hyperlipemia, we took fasting blood samples at 2 and 4 month intervals. The subjects then began taking 1,500 to 2,000mg per day of the hydrogen emitting CCH supplements, divided into twice-daily doses. Fasting blood samples were then taken at 2 and 4 month intervals.

Results
Two months after dietary guidance, neutral lipids went down significantly from 244 ± 19mg/dL to 188 ± 14mg/dL. In the following 2 months, subjects were unable to reach normal lipid levels and no further reductions were realized. Two months after starting on CCH supplements, subjects’ lipid levels fell significantly to 141 ± 12mg/dL and stabilized within the normal range. No significant changes were noted in the levels of HDL, LDL or total cholesterol with respect to dietary guidance or the CCH substance. However, weight was reduced significantly with subjects losing, on average, 1.3kgs following diet guidance and a further 2.1kgs, on average, after taking the CCH supplements. Additionally, subjects with diabetes were all confirmed to have reduced levels of A1c.

Conclusion
In our experiments, the CCH substance significantly lowered the level of neutral lipids and lowered weight. Subjects with diabetes experienced an improvement in their blood sugar level (A1c), which may suggest the effects of CCH influenced improvements in cellular energy metabolism. CCH holds future potential as a useful adjunct to antioxidant therapy and in supporting measures to improve metabolism. This summary was presented at the 106 th Annual Meeting of the Japan Internal Medicine Society (2009).

4. The impact of coral calcium hydride on gene expression and the functional networks of senescence-accelerated mice.

Purpose
The use of senescence accelerated mice /P-8 (SAM/P-8) is well known. Scientific understandings of premature aging point to a breakdown in the balance between oxidation and reduction. We administered the substance known as HydroZen to mice and looked for antioxidant activity that may affect gene expression in the hippocampus.

Method
We administered HydroZen to SAM/P-8 for 10 weeks and analyzed the gene expression of the mice using a DNA microarray method.

Results and Conclusion
Two kinds of gene expression patterns were observed. One was the gene group related to glycolipid metabolism, which had a prominent rise in expression. The other was the gene group related to carcinogenesis and immunity, which showed a decline in expression.